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ÀÛ¼ºÀÚ | °ü¸®ÀÚ | ÀÛ¼ºÀÏ | 2022-04-04 |
¼¿ï´ëÇб³ »ý¹°Á¤º¸ÇÐ Çùµ¿°úÁ¤ ÁÖÃÖ·Î ¼¼¹Ì³ª¸¦ ¾Æ·¡¿Í °°ÀÌ ¿°íÀÚ ÇÏ¿À´Ï ¸¹Àº Âü¿© ¹Ù¶ø´Ï´Ù. ¼¼¹Ì³ª´Â ZoomÀ» ÅëÇÑ ¿Â¶óÀÎ °ÀÇ·Î ¿¹Á¤µÇ¾î ÀÖÀ¸¸ç °ÀÇ¿¡ Âü¿©ÇÏ°íÀÚ ÇϽô ºÐ²²¼´Â ¾Æ·¡ÀÇ ¸µÅ©·Î Âü¿© ºÎŹµå¸®°Ú½À´Ï´Ù. ÀϽÃ: 2022³â 4¿ù 7ÀÏ ¸ñ¿äÀÏ ¿ÀÀü 11½Ã ¿¬»ç: ±èÇüÇ¥ ¿¬»ç´Ô (¿¬¼¼´ëÇб³) Zoom link:¡¡https://snu-ac-kr.zoom.us/j/95898298596 Title: Deciphering the role of CTCF in three-dimensional enhancer network ABSTRACT CTCF is crucial to the organization of mammalian genomes into loop structures. According to recent studies, the transcription apparatus is compartmentalized and concentrated at super-enhancers to form phase-separated condensates and drive the expression of cell-identity genes. However, it remains unclear whether and how transcriptional condensates are coupled to higher-order chromatin organization. Here, we show that CTCF is essential for RNA polymerase II (Pol II)-mediated chromatin interactions, which occur as hyperconnected spatial clusters at super-enhancers. We also demonstrate that CTCF clustering, unlike Pol II clustering, is independent of liquid-liquid phase-separation and resistant to perturbation of transcription. Interestingly, clusters of Pol II, BRD4, and MED1 were found to dissolve upon CTCF depletion, but were reinstated upon restoration of CTCF, suggesting a potent instructive function for CTCF in the formation of transcriptional condensates. Overall, we provide evidence suggesting that CTCF-mediated chromatin looping acts as an architectural prerequisite for the assembly of phase-separated transcriptional condensates.
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