제목	2021년 9월 9일 생물정보학 세미나
작성자	관리자	작성일	2021-09-02
서울대학교 생물정보학 협동과정 주최로 세미나를 아래와 같이 열고자 하오니 많은 참여 바랍니다. 세미나는 Zoom을 통한 온라인 강의로 예정되어 있으며 강의에 참여하고자 하시는 분께서는 아래의 링크로 참여 부탁드리겠습니다. 일시: 2021년 9월 9일 목요일 오전 11시 연사: 김준일 연사님 (숭실대학교) Zoom link:　https://snu-ac-kr.zoom.us/j/83398684837 Title:　Neighbor-specific gene expression revealed from physically interacting cells during mouse embryonic development Abstract Cells continuously communicate with the neighboring cells during development. Direct interaction of different cell types can induce molecular signals dictating lineage specification and cell fate decisions.　 The current single cell RNAseq (scRNAseq) technology cannot study cell contact dependent gene expression due to the loss of spatial information. To overcome this issue and determine cell contact specific gene expression during embryogenesis, we performed RNA sequencing of physically interacting cells (PICseq) and assessed alongside our single cell transcriptomes (scRNAseq) derived from developing mouse embryos between embryonic day (E) 7.5 and E9.5. Analysis of PICseq data identifies an interesting suite of gene expression signatures depending on neighboring cell types. For instance, neural progenitor (NP) cells expressed Nkx2-1 when interacting with definitive endoderm (DE) and DE cells expressed Gsc when interacting with NP. Based on the identified cell contact specific genes, we devised a means to predict the neighboring cell types from individual cell transcriptome. We further developed spatial-tSNE to show the pseudo-spatial distribution of cells in a 2-dimensional space. In sum, we suggest an approach to study contact specific gene regulation during embryogenesis.