서울대학교 생물정보학 협동과정 주최로 세미나를 아래와 같이 열고자 하오니 많은 참여 바랍니다.

일시:　　2019년 11월 12일 화요일 오전 11시

연사:　 장혜식 교수님　(서울대학교)

장소:　　서울대학교　300동 (유회진 학술정보관) 611호

TITLE

Single-molecule RNA analysis with nanopore sequencing

ABSTRACT

　The regulatory elements in RNA give rise to the specificity of the regulations that it receives. As a result, depending on the context, an RNA molecule forms different secondary structures,　binds to different proteins, and gains different chemical modifications or tails. Since the introduction of RNA-Seq to the field, newly developed high-throughput techniques have driven　the massive transcriptome-wide discovery of novel RNA regulations. However, two common　drawbacks in the second generation sequencers have prohibited analyzing the status of　regulatory sites in the broader context of the transcripts. The first problem comes from the　indispensable reverse-transcription, and another is from the short read　lengths. Nanopore sequencing is an entirely different approach that is free from those limitations. It reads the nucleic acid sequences by measuring ionic current changes during their　migrations through a tiny hole in the middle of a transmembrane protein. This alternative　strategy makes it possible to directly monitor the chemical details of the bases throughout the　full lengths of RNA molecules one by one. We are carrying out examinations how nanopore direct RNA sequencing adds new dimensions of the understandings across the　life cycle of RNA. In this talk, I will briefly discuss the preliminary results from our first trials, which include poly(A) tail length measurement, transcriptome timestamping, multiplexed　sequencing, targeted sequencing, secondary structure probing, and base modification　detection.